PEMEKATAN HIDROLISAT DEDAK SORGUM (Sorghum bicolor L Moench) B-100 HASIL HIDROLISIS KULTUR Rhizopus C1 MELALUI ULTRAFILTRASI SEL BERPENGADUK UNTUK ANTI KOLESTEROL

Agustine Susilowati, Aspiyanto Aspiyanto, Y M Iskandar

Abstract


Telah dilakukan pemekatan hidrolisat dedak sorgum (Sorghum bicolor L Moench) B-100 hasil hidrolisis kultur Rhizopus C1,melalui ultrafiltrasi (UF) sel berpengaduk untuk memperoleh SDF/soluble dietary fiber sebagai anti kolesterol. Ultrafitrasi dilakukan pada tekanan 30 dan 40 psia dengan kecepatan putar pengaduk yaitu 200, 300 dan 400 rpm. Hasil penelitian menunjukkan bahwa kondisi operasi pemekatan berpengaruh terhadap fluks permeat dan komposisi retentat & permeat. Perlakuan optimal berdasarkan SDF tertinggi dicapai pada tekanan 40 psia dengan kecepatan putar 300 rpm yang menghasilkan konsentrat dengan komposisi SDF 2,1575% (b.k), total padatan 1,8017 %, protein terlarut 0,0492 mg/mL, total protein 1,432 (b.k), tanin  0,043 % dan gula reduksi 1,744 mg/mL pada fluks 1,5 mL/cm2. menit.

Keywords


SDF; dedak sorgum; ultrafiltrasi; hidrolisis; retentat

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References


Anonimous, 2001. "The Definition of Dietary Fiber", American Association of Cereal Chemists, in Cereal Foods World 46: 112-126, www.aaccnet.org/news/pdfs/DFDef.pdf.

Anonim, 2002. Katalog dan Manual Stirred Ultrafiltration Cells, Amicon.

Anonim. 2003. Tentang Serat Makanan, Gizi Net, Indonesia Nutrition Network, KQMIS 12

Anonymous. 1995. Official Methods of Analysis of the Association of Analytical Chemistry, AOAC Inc., Washington D.C.

Anonim. 2005. Membrane Technology For Process Industry, http:www.pcims.com/images/TP 105.5us.pdf; PCI Membrane System Inc. Milford, USA.

Awika, J.M. & Rooney, L.W. 2004, ‘Sorghum phytochemicals and their potential impact on human health’, Phytochemistry, vol. 65, pp. 1199–1221.

Cheryan, M. 1992. Membrane Technology in Food Bioprocessing, Didalam R. P. Singh dan M. A. Wirakartakusumah (eds.), Advances in Food Engineering, CRC Press Inc., Boca Ratan, Florida.

C. Anglani. 1998. Sorgum carbohydrates - A review, Journal of Plant Foods for Human Nutrition 52 : 77 - 83, 1998. c, Kluwer Academic Publishers, Printed in the Nederlands.

Fellows, P. J. 1992. Food Processing Technology : Principles and Practices, Ellis Hordwood, New York.

Human, S., 2006. Pemuliaan Tanaman Sorghum di PATIR-BATAN. http://www.batan.go.id/patir/berita/ pertanian/sorgum/sorgum.htm.

Iskandar, Y.M, et al, 2002., Pembuatan inokulum menggunakan isolat Rhizopus C1 dan Rhizopus C2 pada substrat campuran, Prosiding Seminar Nasional Tantangan Penelitian Kimia Dalam Era Biologi dan Super Informasi, Pusat Penelitian Kimia - LIPI, ISBN 979-9477-69-7, Jakarta, 17 September .

Karos, W.J. , Ma, Y.H., Shimidzu, T. 1996. Terminology for Membranes and Membranes Processes (IUPAC Recommendations 1996). Pure & Appl. Che. 68: 1479-1489.

Kulp, K. 1975. Carbohydrates. Di dalam: Reed, G. (ed.). Enzymes in Food Processing. Academic Press, New York. Liu, S. 2006. New Techniques for Tea Catechins Extraction. International Traning Workshop of Tea Science, Hunan Agricultural University, Changsa, Hunan, P. R. China, 21 Juli - 10 Agustus.

Linder, M. C. 2002. Biokimia Nutrisi dan Metabolisme, Aminuddin Parakkasi (Penerjemah), A. Y. Amwila (Pendamping), Penerbit Universitas Indonesia, hal. 51-56.

Mulder, M. H. V. 1996. Basic Principles of Membrane Technology, Kluwer Academic Publishers, Dordecht.

Michael, A. S. 1989. Handbook of Industrial Membrane Technology, Noyes Publications, Park Ridge, U.S.A.

Nagodawithana, T. 1994. Savory Flavors, in Alan G., Bioprocess, Fragrance and Color Ingredients, John Wiley & Sons, Inc., New York.

Porsky, et all, 1998, Comparative Chemical Analysis of Fibre Material Prepared by Enzymatic and Chemical Methods from Two Mushrooms (Pleoratus sajor-caju and Pleoratus tuber regium) dalam Cheung, P.C.K and M.Y. Lee, J.Agriculture Food. Chem. 46; 4854-4857 .

Paulson, D.J. 1995. Membranes, the Finest Filtration . By : Introductionto crossflow Membranes Technology.Published in Filtration News. http ://www.enviromentalexpert.com/articles/article 11/ article 11.htm.

Raja Ghosh. 2003. Protein Bioseparation Using Ultrafiltration : Theory, Applications and New Developments, Imperial College Press, London, 105 – 109.

Susilowati, A, dkk. 2010. Pengembangan Pangan Fungsional berbasis polisakarida dari sorgum (Sorgum bicolor L Moench) untuk anti kolesterol, Laporan Hasil Penelitian, Program Tematik, Kedeputian IPT, Tahun Anggaran 2010, Pusat Penelitian Kimia - LIPI, Kawasan PUSPIPTEK, Serpong, Tangerang.

Saba, W.J., W.H. Hale and Theurer. 1972. In vitro rumen fermentaton studies with a bird resistant sorghum grain. Journal of Animal Science. 35 (5): 1076-1082.


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